Celluclast 2L, Novozyme 188

Optimization of temperature and enzyme concentration in the enzymatic saccharification of steam-pretreated wil junior-grade Robert Eklund, Mats Galbe and Guido Zacchi chemical substance engine room I, University of Lund, Lund, Sweden The effect of temperature and enzyme concentration on the enzymatic hydrolysis of steam-pretreated willow was investigated to accommodate the optimal hydrolysis conditions. The willow was pretreated at 220°C for 10 come down according to the results of earlier optimisation studies of the steam-pretreatment conditions for high glucose regainings. The concentrations of two technical enzymes, Celluclast 2L and Novozyme 188, were varied in the pasture 0-20% (based on oven-dry subst govern). A maximum in the conversion o f cellulose to glucose was obtained using a mixture of 15% Celluclast and 3% Novozyme, corresponding to an activity of 11.4 FPU g-~ subst esteem. higher(prenominal)(prenominal) enzyme concentrations did not increase the sign hy drolysis rate nor affect the utmost glucose yield. The effects of temperature in the range 40°C to 60°C were investigated. The temperature affects both the initial hydrolysis rate and the final glucose yield. The highest glucose yield was obtained at 40°C.

The yield change magnitude with increase temperature, collect to increased enzyme deactivation at higher temperatures, while the initial reaction rate increased with increasing temperatures. Keywords: Enzymes; saccharification; steam-pretreatment; willow; hydrolysis; temperature Introduction The make up of rough somatic and enzymes are the two major follow components in th e bioconversion of lignocellulosic literal ! to ethanol. =In a previous study,2 the cost of enzymes were found to require about 80% of the total hydrolysis cost. It is gum olibanum unavoidable to optimize the enzymatic hydrolysis to obtain high yields at low enzyme consumption. In two previous studies, 3,4 steam pretreatment was shown to be a very efficient method of making the raw material, willow, approachable for enzymatic saccharification. Glucose...If you want to get a all-embracing essay, set up it on our website: BestEssayCheap.com

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